Differential expression of immunologic proteins in gingiva after socket preservation in mini pigs

UNLABELLED During healing following tooth extraction, inflammation and the immune response within the extraction socket are related to bone resorption. OBJECTIVE We sought to identify how the alloplastic material used for socket preservation affects the immune responses and osteoclastic activity within extraction sockets. MATERIAL AND METHODS Using a porcine model, we extracted teeth and grafted biphasic calcium phosphate into the extraction sockets. We then performed a peptide analysis with samples of gingival tissue from adjacent to the sockets and compared the extraction only (EO) and extraction with socket preservation (SP) groups. We also used real-time polymerase chain reaction (PCR) to evaluate the expression level of immunoglobulins, chemokines and other factors related to osteoclastogenesis. Differences between the groups were analyzed for statistical significance using paired t tests. RESULTS Levels of IgM, IgG and IGL expression were higher in the EO group than in the SP group 1 week post-extraction, as were the levels of CCL3, CCL5, CXCL2, IFN-γ and TNF-α expression (p < 0.05). In addition, receptor activator of nuclear factor kappa-B ligand (RANKL) was also significantly upregulated in the EO group (p < 0.05), as were IL-1β, IL-6 and IL-8 (p < 0.05). CONCLUSIONS These results suggest that the beneficial effect of socket preservation can be explained by suppression of immune responses and inflammation.